Towards the Establishment of a Biomedical Ontology for the Primary Sjögren’s Syndrome.

Rheumatism represents any illness situation marked with irritation and ache within the joints, muscle tissues, or connective tissues. Many conventional Chinese language medication have been used for a very long time to deal with rheumatism. Nonetheless, a complete data supply for these medication continues to be lacking, and their anti-rheumatism mechanisms stay unclear. An ontology for anti-rheumatism conventional Chinese language medication would strongly assist the illustration, evaluation, and understanding of those medication.

On this research, we first systematically collected reported details about 26 conventional Chinese language decoction items medication, together with their chemical components and adversarial occasions (AEs). By largely reusing phrases from current ontologies (e.g., TCMDPO for conventional Chinese language medicines, NCBITaxon for taxonomy, ChEBI for chemical parts, and OAE for adversarial occasions) and making semantic axioms linking totally different entities, we developed the Ontology of Chinese language Medication for Rheumatism (OCMR) that features over 3000 class phrases.

Our OCMR evaluation discovered that these 26 conventional Chinese language decoction items are made out of anatomic entities (e.g., root and stem) from three Bilateria animals and 23 Mesangiospermae vegetation. Anti-inflammatory and antineoplastic roles are essential for anti-rheumatism medication. Utilizing the entire of 555 distinctive ChEBI chemical entities recognized from these medication, our ChEBI-based classification evaluation recognized 18 anti-inflammatory, 33 antineoplastic chemical substances, and 9 chemical substances (together with three diterpenoids and three triterpenoids) having each anti-inflammatory and antineoplastic roles.

Moreover, our research detected 22 diterpenoids and 23 triterpenoids, together with 16 pentacyclic triterpenoids which might be seemingly bioactive towards rheumatism. Six medication have been discovered to be related to 184 distinctive AEs, together with three AEs (i.e., dizziness, nausea and vomiting, and anorexia) every related to 5 medication. A number of chemical entities are labeled as neurotoxins (e.g., diethyl phthalate) and allergens (e.g., eugenol), which can clarify the formation of some TCD AEs. The OCMR might be effectively queried for helpful data utilizing SPARQL scripts.

The OCMR ontology was developed to systematically symbolize 26 conventional anti-rheumatism Chinese language medication and their associated data. The OCMR evaluation recognized attainable anti-rheumatism and AE mechanisms of those medication. Our novel ontology-based strategy can be utilized to systematic illustration and evaluation of different conventional Chinese language medication.

In biomedicine, high-quality metadata are essential for locating experimental datasets, for understanding how experiments have been carried out, and for reproducing these experiments. Regardless of the current deal with metadata, the standard of metadata accessible in public repositories continues to be extraordinarily poor. A key issue is that the standard metadata acquisition course of is time-consuming and error susceptible, with weak or nonexistent assist for linking metadata to ontologies.

There’s a urgent want for strategies and instruments to hurry up the metadata acquisition course of and to extend the standard of metadata which might be entered. On this paper, we describe a technique and set of related instruments that we developed to deal with this problem.

Reticulocalbin 1 (RCN1) Antibody
20-abx126465	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

RCN1 Polyclonal Conjugated Antibody
C27480	SAB	100ul	EUR 397

RCN1 cloning plasmid
CSB-CL618980HU-10ug	Cusabio	10ug	EUR 233
Description: A cloning plasmid for the RCN1 gene.

RCN1 Rabbit pAb
A10572-100ul	Abclonal	100 ul	EUR 308

RCN1 Rabbit pAb
A10572-200ul	Abclonal	200 ul	EUR 459

RCN1 Rabbit pAb
A10572-20ul	Abclonal	20 ul	EUR 183

RCN1 Rabbit pAb
A10572-50ul	Abclonal	50 ul	EUR 223

Human Reticulocalbin-1 (RCN1)
1-CSB-YP618980HU	Cusabio	EUR 430.00 EUR 234.00 EUR 1508.00 EUR 642.00 EUR 1009.00 EUR 291.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Reticulocalbin-1(RCN1),partial expressed in Yeast

Mouse RCN1 shRNA Plasmid
20-abx972413	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg

Human RCN1 shRNA Plasmid
20-abx954030	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg

Reticulocalbin 1 (RCN1) Protein
20-abx262856	Abbexa	EUR 328.00 EUR 6397.00 EUR 230.00	10 ug 1 mg 2 µg

RCN1 ELISA KIT|Human
EF007140	Lifescience Market	96 Tests	EUR 689

Anti-RCN1 (1F8-E4)
YF-MA15139	Abfrontier	100 ug	EUR 363
Description: Mouse monoclonal to RCN1

Anti-RCN1 (4D9-G3)
YF-MA15140	Abfrontier	100 ug	EUR 363
Description: Mouse monoclonal to RCN1

RCN1 Recombinant Protein (Human)
RP026077	ABM	100 ug	Ask for price

RCN1 Recombinant Protein (Mouse)
RP167399	ABM	100 ug	Ask for price

RCN1 Recombinant Protein (Rat)
RP223949	ABM	100 ug	Ask for price

RCN1 protein (His tag)
80R-1434	Fitzgerald	50 ug	EUR 305
Description: Purified recombinant Human RCN1 protein

RCN1 ELISA Kit (Human) (OKEI00324)
OKEI00324	Aviva Systems Biology	96 Wells	EUR 767
Description: Description of target: May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment. ;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.375 ng/mL

Rcn1 ORF Vector (Rat) (pORF)
ORF074651	ABM	1.0 ug DNA	EUR 506

RCN1 ORF Vector (Human) (pORF)
ORF008693	ABM	1.0 ug DNA	EUR 95

Rcn1 ORF Vector (Mouse) (pORF)
ORF055801	ABM	1.0 ug DNA	EUR 506

RCN1 Reticulocalbin 1 Human Recombinant Protein
PROTQ15293	BosterBio	Regular: 10ug	EUR 317
Description: RCN1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 341 amino acids (30-331 a.a.) and having a molecular mass of 40.4kDa (real molecular weight on SDS-PAGE will be shift up). The RCN1 is fused to 39 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Human Reticulocalbin 1 (RCN1)ELISA Kit
201-12-2939	SunredBio	96 tests	EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Reticulocalbin 1 (RCN1) ELISA Kit
abx253097-96tests	Abbexa	96 tests	EUR 707

Human Reticulocalbin 1(RCN1)ELISA Kit
QY-E01114	Qayee Biotechnology	96T	EUR 361

Human RCN1(Reticulocalbin 1) ELISA Kit
EH3705	FN Test	96T	EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.375 ng/ml

Mouse Reticulocalbin- 1, Rcn1 ELISA KIT
ELI-41186m	Lifescience Market	96 Tests	EUR 865

Human Reticulocalbin- 1, RCN1 ELISA KIT
ELI-18302h	Lifescience Market	96 Tests	EUR 824

RCN1 sgRNA CRISPR Lentivector set (Human)
K1803801	ABM	3 x 1.0 ug	EUR 339

Rcn1 sgRNA CRISPR Lentivector set (Mouse)
K4781201	ABM	3 x 1.0 ug	EUR 339

Rcn1 sgRNA CRISPR Lentivector set (Rat)
K6523601	ABM	3 x 1.0 ug	EUR 339

ELISA kit for Human RCN1 (Reticulocalbin 1)
E-EL-H5569	Elabscience Biotech	1 plate of 96 wells	EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Human RCN1 (Reticulocalbin 1) in samples from Serum, Plasma, Cell supernatant

RCN1 sgRNA CRISPR Lentivector (Human) (Target 1)
K1803802	ABM	1.0 ug DNA	EUR 154

RCN1 sgRNA CRISPR Lentivector (Human) (Target 2)
K1803803	ABM	1.0 ug DNA	EUR 154

RCN1 sgRNA CRISPR Lentivector (Human) (Target 3)
K1803804	ABM	1.0 ug DNA	EUR 154

Rcn1 sgRNA CRISPR Lentivector (Mouse) (Target 1)
K4781202	ABM	1.0 ug DNA	EUR 154

Rcn1 sgRNA CRISPR Lentivector (Mouse) (Target 2)
K4781203	ABM	1.0 ug DNA	EUR 154

Rcn1 sgRNA CRISPR Lentivector (Mouse) (Target 3)
K4781204	ABM	1.0 ug DNA	EUR 154

Rcn1 sgRNA CRISPR Lentivector (Rat) (Target 1)
K6523602	ABM	1.0 ug DNA	EUR 154

Rcn1 sgRNA CRISPR Lentivector (Rat) (Target 2)
K6523603	ABM	1.0 ug DNA	EUR 154

Rcn1 sgRNA CRISPR Lentivector (Rat) (Target 3)
K6523604	ABM	1.0 ug DNA	EUR 154

RCN1 Protein Vector (Rat) (pPB-C-His)
PV298602	ABM	500 ng	EUR 603

RCN1 Protein Vector (Rat) (pPB-N-His)
PV298603	ABM	500 ng	EUR 603

RCN1 Protein Vector (Rat) (pPM-C-HA)
PV298604	ABM	500 ng	EUR 603

RCN1 Protein Vector (Rat) (pPM-C-His)
PV298605	ABM	500 ng	EUR 603

RCN1 Protein Vector (Human) (pPB-C-His)
PV034769	ABM	500 ng	EUR 329

RCN1 Protein Vector (Human) (pPB-N-His)
PV034770	ABM	500 ng	EUR 329

RCN1 Protein Vector (Human) (pPM-C-HA)
PV034771	ABM	500 ng	EUR 329

RCN1 Protein Vector (Human) (pPM-C-His)
PV034772	ABM	500 ng	EUR 329

RCN1 Protein Vector (Mouse) (pPB-C-His)
PV223202	ABM	500 ng	EUR 603

RCN1 Protein Vector (Mouse) (pPB-N-His)
PV223203	ABM	500 ng	EUR 603

RCN1 Protein Vector (Mouse) (pPM-C-HA)
PV223204	ABM	500 ng	EUR 603

RCN1 Protein Vector (Mouse) (pPM-C-His)
PV223205	ABM	500 ng	EUR 603

RCN1 3'UTR GFP Stable Cell Line
TU069702	ABM	1.0 ml	EUR 1394

Rcn1 3'UTR Luciferase Stable Cell Line
TU217430	ABM	1.0 ml	Ask for price

RCN1 3'UTR Luciferase Stable Cell Line
TU019702	ABM	1.0 ml	EUR 1394

Rcn1 3'UTR GFP Stable Cell Line
TU267430	ABM	1.0 ml	Ask for price

Rcn1 3'UTR Luciferase Stable Cell Line
TU117670	ABM	1.0 ml	Ask for price

Rcn1 3'UTR GFP Stable Cell Line
TU167670	ABM	1.0 ml	Ask for price

RCN1 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)
LV647263	ABM	1.0 ug DNA	EUR 514

RCN1 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)
LV647267	ABM	1.0 ug DNA	EUR 514

RCN1 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)
LV647268	ABM	1.0 ug DNA	EUR 514

RCN1 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)
LV792577	ABM	1.0 ug DNA	EUR 316

RCN1 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a)
LV792578	ABM	1.0 ug DNA	EUR 316

Recombinant Saccharomyces Cerevisiae RCN1 Protein (aa 1-211) [His]
VAng-Wyb5435-1mg	Creative Biolabs	1 mg	EUR 4054
Description: Saccharomyces Cerevisiae (strain ATCC 204508 / S288c) Rcn1p protein, recombinant protein.

RCN1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)
K1803805	ABM	3 x 1.0 ug	EUR 376

Rcn1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)
K4781205	ABM	3 x 1.0 ug	EUR 376

Rcn1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)
K6523605	ABM	3 x 1.0 ug	EUR 376

RCN1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)
K1803806	ABM	1.0 ug DNA	EUR 167

RCN1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)
K1803807	ABM	1.0 ug DNA	EUR 167

RCN1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)
K1803808	ABM	1.0 ug DNA	EUR 167

RCN1 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)
LV647264	ABM	1.0 ug DNA	EUR 514

RCN1 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)
LV647265	ABM	1.0 ug DNA	EUR 572

RCN1 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
LV647266	ABM	1.0 ug DNA	EUR 572

Rcn1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)
K4781206	ABM	1.0 ug DNA	EUR 167

Rcn1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)
K4781207	ABM	1.0 ug DNA	EUR 167

Rcn1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)
K4781208	ABM	1.0 ug DNA	EUR 167

Rcn1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)
K6523606	ABM	1.0 ug DNA	EUR 167

Rcn1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)
K6523607	ABM	1.0 ug DNA	EUR 167

Rcn1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)
K6523608	ABM	1.0 ug DNA	EUR 167

RCN1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
LV792576	ABM	1.0 ug DNA	EUR 374

CD11b Antibody Antibody
ABD2911	Lifescience Market	100 ug	EUR 438

H2B Antibody Antibody
AF4659	Affbiotech	200ul	EUR 376
Description: H2B Antibody Antibody detects endogenous levels of H2B.

anti- Antibody^Polyclonal antibody control antibody
LSMab09882	Lifescience Market	100 ug	EUR 438

Antibody
A1360-500	Biovision		Ask for price

Anti-Anti-SEPT2 Antibody antibody
STJ25475	St John's Laboratory	100 µl	EUR 277

Anti-Anti-SEPT5 Antibody antibody
STJ25477	St John's Laboratory	100 µl	EUR 277
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-SEPT8 Antibody antibody
STJ25479	St John's Laboratory	100 µl	EUR 277
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT2 Antibody antibody
STJ28365	St John's Laboratory	100 µl	EUR 277

Anti-Anti-SEPT7 Antibody antibody
STJ28963	St John's Laboratory	100 µl	EUR 277
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Anti-Anti-SEPT8 Antibody antibody
STJ117206	St John's Laboratory	100 µl	EUR 277
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT12 Antibody antibody
STJ117759	St John's Laboratory	100 µl	EUR 277
Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-MARCH6 Antibody antibody
STJ118549	St John's Laboratory	100 µl	EUR 277

A core element of this strategy is a price advice framework that makes use of evaluation of beforehand entered metadata and ontology-based metadata specs to assist customers quickly and precisely enter their metadata. We carried out an preliminary analysis of this strategy utilizing metadata from a public metadata repository.